Widespread, specific binding of 25-hydroxycholecalciferol in rat tissues.
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چکیده
منابع مشابه
Competitive protein-binding assay for 25-hydroxycholecalciferol.
1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 5 SD 14 pmol/ml (1 5.2 & 5.6 nglml). 3. Levels of 25-hydroxycholecalciferol...
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Methods: The prevalence of multiple sclerosis (MS) is high in patients suffering from 25-hydroxyvitamin D3 [25(OH)D] deficiency. The aim of this study was to investigate the possible association of saliva and serum 25(OH)D concentrations with MS in women.Methods: Serum and saliva 25(OH)D levels of 30 MS women and 30 matched healthy controls in this case-control study were measured by ELIS...
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Intact diaphragms from vitamin D-deficient rats were incubated in vitro with [3H]leucine. Oral administration of 10 mug (400 U) of cholecalciferol 7 h before incubation increased leucine incorporation into diaphragm muscle protein by 136% (P less than 0.001) of the preparation from untreated animals. Nephrectomy did not obliterate this response. ATP content of the diaphragm muscle was also enha...
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(23S)-23,25-Dihydroxycholecalciferol was converted into at least five metabolites in kidney homogenates prepared from 1,25-dihydroxycholecalciferol-treated chickens. One of these has been positively identified as 23,25,26-trihydroxycholecalciferol by u.v.-absorbance analysis, mass spectrometry and chemical formation of derivatives. 23,25,26-Trihydroxycholecaciferol produces 25-hydroxycholecalci...
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In vitro evidence presently favors a direct osteolytic effect of biologically active vitamin D metabolites. Studies were designed to evaluate the effect of 25-hydroxycholecalciferol (25OHD3) on bone collagen and mineral maturation in vivo and its dependence on parathyroid hormone (PTH). After treatment of sham-operated control and parathyroidectomized (PTX) mature rats with either 25OHD3 or an ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)42014-0